The objective of this study is to quantify the effects of mesenchymal stem cell (MSC) treatment on experimental abdominal aortic aneurysm (AAA) expansion. Accumulating evidence implicates activation of lymphocytes as an inciting event in an inflammatory cascade that leads to aneurysm formation and expansion. This suggests that an agent capable of attenuating lymphocyte activation may prove effective in preventing aneurysm formation and expansion. MSCs from bone marrow and adipose tissue have demonstrated immunomodulatory properties both in vitro and in vivo. Thus, we propose to combine a commonly used murine AAA model (angiotensin II or angII) with MSC injections after aneurysm formation for this study. To fully characterize the development and progression of these aneurysms, we will use non-invasive three dimensional ultrasound imaging. This advanced technique provides mouse-specific information, reducing the number of animals required and giving us the ability to follow aortic wall size, dynamics, and composition in longitudinal studies. We expect that the injection of MSCs will significantly attenuate angII-induced AAA expansion.
|Program type||Undergraduate Fellowship|
|Effective start/end date||06/01/2015 → 08/31/2015|