The overall objective of this proposal is to use a new technique--microfluidic 3-D printing--to engineer myocardial tissue constructs (MTCs) using cardiac myocytes obtained by differentiation of human induced pluripotent stem cells (hIPSC-CMs). We hypothesize that longitudinal/anisotropic alignment in three dimensions will promote sarcomere organization as well as metabolic and electrophysiologic maturation of hIPSC-CMs to the adult stage of cardiac development. Once established as an effective in vitro model, MTCs will be used to test whether reducing myofilament Ca2+ sensitivity prevents activation of hypertrophic signaling in patient-derived hIPSC-CMs expressing a mutation that leads to hypertrophic cardiomyopathy (HCM).
|Program type||Innovative Research Grant|
|Effective start/end date||01/01/2017 → 12/31/2018|