Microfluidic 3-D Printing of Myocardial Tissue Constructs to Examine the Metabolic Basis of Hypertrophic Cardiomyopathy

Project: Research

Investigators

  • Maria Roselle Abraham (PI)

Description

The overall objective of this proposal is to use a new technique--microfluidic 3-D printing--to engineer myocardial tissue constructs (MTCs) using cardiac myocytes obtained by differentiation of human induced pluripotent stem cells (hIPSC-CMs). We hypothesize that longitudinal/anisotropic alignment in three dimensions will promote sarcomere organization as well as metabolic and electrophysiologic maturation of hIPSC-CMs to the adult stage of cardiac development. Once established as an effective in vitro model, MTCs will be used to test whether reducing myofilament Ca2+ sensitivity prevents activation of hypertrophic signaling in patient-derived hIPSC-CMs expressing a mutation that leads to hypertrophic cardiomyopathy (HCM).
Award amount$149,562.00
Award date01/01/2017
Program typeInnovative Research Grant
Award ID17IRG33460364
Effective start/end date01/01/201712/31/2018
StatusFinished