ADAMTS Proteins: Concepts, Challenges, and Prospects

Research output: Chapter in Book/Report/Conference proceedingChapter

Details

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
StatusPublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2043
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

The ADAMTS superfamily comprises secreted metalloproteases (ADAMTS proteases) as well as structurally related secreted glycoproteins that lack catalytic activity (ADAMTS-like proteins). Members of both families participate in diverse morphogenetic processes during embryonic development, and connective tissue maintenance and hemostasis in the adult. Several ADAMTS proteins are heavily implicated in genetic and acquired human and animal disorders. Despite these indicators of a profound biological and medical importance, detailed knowledge about their molecular structures, substrates, biological pathways, and biochemical mechanisms is significantly limited by unique intrinsic characteristics, which have led to several technical challenges. As a group, they are larger, more heavily modified, and harder to purify than other secreted proteases. In addition, idiosyncratic aspects of individual members are deserving of further investigation but can complicate their analysis. Here, some of the key concepts, challenges, and prospects in ADAMTS research are discussed in the context of the knowledge accumulated over the past two decades. Individual chapters in this volume of Methods in Molecular Biology provide practical solutions for surmounting these challenges. Since the biology of a protease is actually the biology of its substrates, there is considerable emphasis on purification of recombinant ADAMTS proteins, identification of their substrates and assays for their proteolytic activity.

    Research areas

  • Degradome, Extracellular matrix, Metalloprotease, Metalloproteinase, Protease, Proteolysis

Citation formats

APA

Apte, S. S. (2020). ADAMTS Proteins: Concepts, Challenges, and Prospects. In Methods in Molecular Biology (pp. 1-12). (Methods in Molecular Biology; Vol. 2043). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9698-8_1

Harvard

Apte, SS 2020, ADAMTS Proteins: Concepts, Challenges, and Prospects. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2043, Humana Press Inc., pp. 1-12. https://doi.org/10.1007/978-1-4939-9698-8_1