Cellular mechanism of premature ventricular contraction–induced cardiomyopathy

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • Virginia Commonwealth University
  • VA Medical Center

Details

Original languageEnglish (US)
Pages (from-to)2064-2072
Number of pages9
JournalHeart Rhythm
Volume11
Issue number11
StatusPublished - Nov 2014
Peer-reviewedYes

Abstract

Background Frequent premature ventricular contractions (PVCs) are associated with increased risk of sudden cardiac death and can cause secondary cardiomyopathy. Objective We sought to determine the mechanism(s) responsible for prolonged refractory period and left ventricular (LV) dysfunction demonstrated in our canine model of PVC-induced cardiomyopathy. Methods Single myocytes were isolated from LV free wall of PVC and control canines and used for patch-clamp recording, intracellular Ca2+ measurements, and immunocytochemistry/confocal microscopy. LV tissues adjacent to the area of myocyte isolation were used for the immunoblot quantification of protein expression. Results In the PVC group, LV ejection fraction decreased from 57.6% ± 1.5% to 30.4% ± 3.1% after ≥4 months of ventricular bigeminy. Compared to control myocytes, PVC myocytes had decreased densities of both outward (transient outward current [Ito] and inward rectifier current [IK1]) and inward (L-type Ca current [ICaL]) currents, but no consistent changes in rapid or slow delayed rectifier currents. The reduction in Ito, IK1, and ICaL was accompanied by decreased protein levels of their channel subunits. The extent of reduction in Ito, IK1, and ICaL varied among PVC myocytes, creating marked heterogeneity in action potential configurations and durations. PVC myocytes showed impaired Ca-induced Ca release from the sarcoplasmic reticulum (SR), without increase in SR Ca leak or decrease in SR Ca store. This was accompanied by a decrease in dyad scaffolding protein, junctophilin-2, and loss of Cav1.2 registry with Ca-releasing channels (ryanodine receptor 2). Conclusion PVCs increase dispersion of action potential configuration/duration, a risk factor for sudden cardiac death, because of the heterogeneous reduction in Ito, IK1, and ICaL. The excitation-contraction coupling is impaired because of the decrease in ICaL and Cav1.2 misalignment with respect to ryanodine receptor 2.

    Research areas

  • Cardiomyopathy, Electrical remodeling, Excitation-contraction coupling, Premature ventricular contraction

Citation formats

APA

Wang, Y., Eltit, J. M., Kaszala, K., Tan, A., Jiang, M., Zhang, M., ... Huizar, J. F. (2014). Cellular mechanism of premature ventricular contraction–induced cardiomyopathy. Heart Rhythm, 11(11), 2064-2072. https://doi.org/10.1016/j.hrthm.2014.07.022

Harvard

Wang, Y, Eltit, JM, Kaszala, K, Tan, A, Jiang, M, Zhang, M, Tseng, GN & Huizar, JF 2014, 'Cellular mechanism of premature ventricular contraction–induced cardiomyopathy', Heart Rhythm, vol. 11, no. 11, pp. 2064-2072. https://doi.org/10.1016/j.hrthm.2014.07.022