Characterization of mitochondrial populations during stem cell differentiation
Research output: Contribution to journal › Article
Mitochondrial dynamics play an important role in numerous physiological and pathophysiological phenomena in the developing and adult human heart. Alterations in structural aspects of cellular mitochondrial composition as a function of changes in physiology can easily be visualized using fl uorescence microscopy. Commonly, mitochondrial location, number, and morphology are reported qualitatively due to the lack of automated and user-friendly computer-based analysis tools. Mitochondrial Quantifi cation using MATLAB (MQM) is a computer-based tool to quantitatively assess these parameters by analyzing fl uorescently labeled mitochondria within the cell; in particular, MQM provides numerical information on the number, area, and location of mitochondria within a cell in a time-effi cient, automated, and unbiased way. This chapter describes the use of MQM’s capabilities to quantify mitochondrial changes during human pluripotent stem cell (hPSC) differentiation into spontaneously contracting cardiomyocytes (SC-CMs), which follows physiological pathways of human heart development.
- Cardiac development, Cardiomyocyte, Fluorescence, MATLAB, Mitochondria, Quantitative analysis