Characterization of mitochondrial populations during stem cell differentiation

Research output: Contribution to journalArticle

Authors

Details

Original languageEnglish (US)
Pages (from-to)453-463
Number of pages11
JournalMethods in Molecular Biology
Volume1264
StatusPublished - Jan 1 2015
Peer-reviewedYes

Abstract

Mitochondrial dynamics play an important role in numerous physiological and pathophysiological phenomena in the developing and adult human heart. Alterations in structural aspects of cellular mitochondrial composition as a function of changes in physiology can easily be visualized using fl uorescence microscopy. Commonly, mitochondrial location, number, and morphology are reported qualitatively due to the lack of automated and user-friendly computer-based analysis tools. Mitochondrial Quantifi cation using MATLAB (MQM) is a computer-based tool to quantitatively assess these parameters by analyzing fl uorescently labeled mitochondria within the cell; in particular, MQM provides numerical information on the number, area, and location of mitochondria within a cell in a time-effi cient, automated, and unbiased way. This chapter describes the use of MQM’s capabilities to quantify mitochondrial changes during human pluripotent stem cell (hPSC) differentiation into spontaneously contracting cardiomyocytes (SC-CMs), which follows physiological pathways of human heart development.

    Research areas

  • Cardiac development, Cardiomyocyte, Fluorescence, MATLAB, Mitochondria, Quantitative analysis

Citation formats

APA

Kerscher, P., Bussie, B. S., Desimone, K. M., Dunn, D. A., & Lipke, E. A. (2015). Characterization of mitochondrial populations during stem cell differentiation. Methods in Molecular Biology, 1264, 453-463. https://doi.org/10.1007/978-1-4939-2257-4_37

Harvard

Kerscher, P, Bussie, BS, Desimone, KM, Dunn, DA & Lipke, EA 2015, 'Characterization of mitochondrial populations during stem cell differentiation', Methods in Molecular Biology, vol. 1264, pp. 453-463. https://doi.org/10.1007/978-1-4939-2257-4_37