Cholesterol acceptors regulate the lipidome of macrophage foam cells

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • Michigan State University
  • Albany Medical College

Details

Original languageEnglish (US)
Article number3784
JournalInternational journal of molecular sciences
Volume20
Issue number15
StatusPublished - Aug 1 2019
Peer-reviewedYes

Abstract

Arterial foam cells are central players of atherogenesis. Cholesterol acceptors, apolipoprotein A-I (apoA-I) and high-density lipoprotein (HDL), take up cholesterol and phospholipids effluxed from foam cells into the circulation. Due to the high abundance of cholesterol in foam cells, most previous studies focused on apoA-I/HDL-mediated free cholesterol (FC) transport. However, recent lipidomics of human atherosclerotic plaques also identified that oxidized sterols (oxysterols) and non-sterol lipid species accumulate as atherogenesis progresses. While it is known that these lipids regulate expression of pro-inflammatory genes linked to plaque instability, how cholesterol acceptors impact the foam cell lipidome, particularly oxysterols and non-sterol lipids, remains unexplored. Using lipidomics analyses, we found cholesterol acceptors remodel foam cell lipidomes. Lipid subclass analyses revealed various oxysterols, sphingomyelins, and ceramides, species uniquely enriched in human plaques were significantly reduced by cholesterol acceptors, especially by apoA-I. These results indicate that the function of lipid-poor apoA-I is not limited to the efflux of cholesterol and phospholipids but suggest that apoA-I serves as a major regulator of the foam cell lipidome and might play an important role in reducing multiple lipid species involved in the pathogenesis of atherosclerosis.

    Research areas

  • ABCA1, ABCG1, Atherosclerosis, Cholesterol, Foam cells, LDL, Lipidome, Lipidomics, Macrophage, Mass-spectrometry, Oxysterols, Reverse cholesterol transport

Citation formats

APA

Paul, A., Lydic, T. A., Hogan, R., & Goo, Y. H. (2019). Cholesterol acceptors regulate the lipidome of macrophage foam cells. International journal of molecular sciences, 20(15), [3784]. https://doi.org/10.3390/ijms20153784

Harvard

Paul, A, Lydic, TA, Hogan, R & Goo, YH 2019, 'Cholesterol acceptors regulate the lipidome of macrophage foam cells', International journal of molecular sciences, vol. 20, no. 15, 3784. https://doi.org/10.3390/ijms20153784