Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice

Research output: Contribution to journalArticle

Authors

  • Charles B. Wright
  • Hironori Uehara
  • Younghee Kim
  • Tetsuhiro Yasuma
  • Reo Yasuma
  • Shuichiro Hirahara
  • Ryan D. Makin
  • Ivana Apicella
  • Felipe Pereira
  • Yosuke Nagasaka
  • Siddharth Narendran
  • Shinichi Fukuda
  • Romulo Albuquerque
  • Benjamin J. Fowler
  • Ana Bastos-Carvalho
  • Philippe Georgel
  • Izuho Hatada
  • Bo Chang
  • Nagaraj Kerur
  • Balamurali K. Ambati
  • Jayakrishna Ambati

External Institution(s)

  • University of Kentucky
  • Loma Linda University
  • University of Virginia
  • OliX Pharmaceuticals, Inc.
  • Universidade Federal de São Paulo
  • Aravind Eye Hospital
  • University of Tsukuba
  • Vistar Eye Center
  • Université de Strasbourg
  • Gunma University
  • Jackson Laboratory

Details

Original languageEnglish (US)
Pages (from-to)2579-2587
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number5
StatusPublished - Feb 4 2020
Peer-reviewedYes

Abstract

Degeneration of the retinal pigmented epithelium (RPE) and aberrant blood vessel growth in the eye are advanced-stage processes in blinding diseases such as age-related macular degeneration (AMD), which affect hundreds of millions of people worldwide. Loss of the RNase DICER1, an essential factor in micro-RNA biogenesis, is implicated in RPE atrophy. However, the functional implications of DICER1 loss in choroidal and retinal neovascularization are unknown. Here, we report that two independent hypomorphic mouse strains, as well as a separate model of postnatal RPE-specific DICER1 ablation, all presented with spontaneous RPE degeneration and choroidal and retinal neovascularization. DICER1 hypomorphic mice lacking critical inflammasome components or the innate immune adaptor MyD88 developed less severe RPE atrophy and pathological neovascularization. DICER1 abundance was also reduced in retinas of the JR5558 mouse model of spontaneous choroidal neovascularization. Finally, adenoassociated vector-mediated gene delivery of a truncated DICER1 variant (OptiDicer) reduced spontaneous choroidal neovascularization in JR5558 mice. Collectively, these findings significantly expand the repertoire of DICER1 in preserving retinal homeostasis by preventing both RPE degeneration and pathological neovascularization.

    Research areas

  • Choroidal neovascularization, Dicer, Inflammasome, Retina

Citation formats

APA

Wright, C. B., Uehara, H., Kim, Y., Yasuma, T., Yasuma, R., Hirahara, S., ... Gelfand, B. D. (2020). Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proceedings of the National Academy of Sciences of the United States of America, 117(5), 2579-2587. https://doi.org/10.1073/pnas.1909761117

Harvard

Wright, CB, Uehara, H, Kim, Y, Yasuma, T, Yasuma, R, Hirahara, S, Makin, RD, Apicella, I, Pereira, F, Nagasaka, Y, Narendran, S, Fukuda, S, Albuquerque, R, Fowler, BJ, Bastos-Carvalho, A, Georgel, P, Hatada, I, Chang, B, Kerur, N, Ambati, BK, Ambati, J & Gelfand, BD 2020, 'Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 5, pp. 2579-2587. https://doi.org/10.1073/pnas.1909761117