DeActs: Genetically encoded tools for perturbing the actin cytoskeleton in single cells

Research output: Contribution to journalArticle

Authors

  • Martin Harterink
  • Marta Esteves Da Silva
  • Lena Will
  • Julia Turan
  • Adiljan Ibrahim
  • Alexander E. Lang
  • Eljo Y. Van Battum
  • R. Jeroen Pasterkamp
  • Lukas C. Kapitein
  • Dmitri Kudryashov
  • Ben A. Barres
  • Casper C. Hoogenraad
  • J. Bradley Zuchero

External Institution(s)

  • Utrecht University
  • Stanford University
  • University of Freiburg

Details

Original languageEnglish (US)
Pages (from-to)479-482
Number of pages4
JournalNature Methods
Volume14
Issue number5
StatusPublished - Apr 27 2017
Peer-reviewedYes

Abstract

The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

Citation formats

APA

Harterink, M., Da Silva, M. E., Will, L., Turan, J., Ibrahim, A., Lang, A. E., ... Zuchero, J. B. (2017). DeActs: Genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nature Methods, 14(5), 479-482. https://doi.org/10.1038/nmeth.4257

Harvard

Harterink, M, Da Silva, ME, Will, L, Turan, J, Ibrahim, A, Lang, AE, Van Battum, EY, Jeroen Pasterkamp, R, Kapitein, LC, Kudryashov, D, Barres, BA, Hoogenraad, CC & Zuchero, JB 2017, 'DeActs: Genetically encoded tools for perturbing the actin cytoskeleton in single cells', Nature Methods, vol. 14, no. 5, pp. 479-482. https://doi.org/10.1038/nmeth.4257