Estrogen receptor profiling and activity in cardiac myocytes

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Colorado Boulder

Details

Original languageEnglish (US)
Pages (from-to)62-70
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume431
StatusPublished - Aug 15 2016
Peer-reviewedYes

Abstract

Estrogen signaling appears critical in the heart. However a mechanistic understanding of the role of estrogen in the cardiac myocyte is lacking. Moreover, there are multiple cell types in the heart and multiple estrogen receptor (ER) isoforms. Therefore, we studied expression, localization, transcriptional and signaling activity of ERs in isolated cardiac myocytes. We found only ERα RNA (but no ERβ RNA) in cardiac myocytes using two independent methods. The vast majority of full-length ERα protein (ERα66) localizes to cardiac myocyte nuclei where it is competent to activate transcription. Alternate isoforms of ERα encoded by the same genomic locus (ERα46 and ERα36) have differential transcriptional activity in cardiac myocytes but also primarily localize to nuclei. In contrast to other reports, no ERα isoform is competent to activate MAPK or PI3K signaling in cardiac myocytes. Together these data support a role for ERα at the level of transcription in cardiac myocytes.

    Research areas

  • Cardiac myocytes, Estradiol, Estrogen, Estrogen receptors

Citation formats

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Pugach, EK, Blenck, CL, Dragavon, JM, Langer, SJ & Leinwand, LA 2016, 'Estrogen receptor profiling and activity in cardiac myocytes', Molecular and Cellular Endocrinology, vol. 431, pp. 62-70. https://doi.org/10.1016/j.mce.2016.05.004