Expression Analysis by RNAscope™ In Situ Hybridization

Research output: Chapter in Book/Report/Conference proceedingChapter

Details

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
StatusPublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2043
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

RNA in situ hybridization has an important place in matrix biology, as the only method that allows for in situ discrimination of precise spatial and temporal patterns of gene expression. Whereas immunohistochemistry shows where a matrix protein localizes, ISH identifies the cell of origin. Thus, these methods provide complementary information for insights on the life cycle of matrix molecules, including ADAMTS proteases. This protocol encompasses the staining of tissue sections to reveal expression of the gene of interest.

    Research areas

  • Gene expression, In situ, RNA, Tissue

Citation formats

APA

Mead, T. J., & Apte, S. S. (2020). Expression Analysis by RNAscope™ In Situ Hybridization. In Methods in Molecular Biology (pp. 173-178). (Methods in Molecular Biology; Vol. 2043). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9698-8_14

Harvard

Mead, TJ & Apte, SS 2020, Expression Analysis by RNAscope™ In Situ Hybridization. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2043, Humana Press Inc., pp. 173-178. https://doi.org/10.1007/978-1-4939-9698-8_14