Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing

Research output: Contribution to journalArticle

Authors

  • Xiaoping Bao
  • Maroof M. Adil
  • Riya Muckom
  • Joshua A. Zimmermann
  • Aurelie Tran
  • Natalie Suhy
  • Yibo Xu
  • Rocío G. Sampayo
  • Douglas S. Clark
  • David V. Schaffer

External Institution(s)

  • University of California at Berkeley
  • Purdue University

Details

Original languageEnglish (US)
Pages (from-to)1556-1566
Number of pages11
JournalSTEM CELLS
Volume37
Issue number12
StatusPublished - Dec 1 2019
Peer-reviewedYes

Abstract

Transcription factors (TFs) are potent proteins that control gene expression and can thereby drive cell fate decisions. Fluorescent reporters have been broadly knocked into endogenous TF loci to investigate the biological roles of these factors; however, the sensitivity of such analyses in human pluripotent stem cells (hPSCs) is often compromised by low TF expression levels and/or reporter silencing. Complementarily, we report an inducible and quantitative reporter platform based on the Cre-LoxP recombination system that enables robust, quantifiable, and continuous monitoring of live hPSCs and their progeny to investigate the roles of TFs during human development and disease. Stem Cells 2019;37:1556–1566.

    Research areas

  • CRISPR/Cas9, Fate mapping, Gene editing, Human pluripotent stem cells, Transcription factors

Citation formats

APA

Bao, X., Adil, M. M., Muckom, R., Zimmermann, J. A., Tran, A., Suhy, N., ... Schaffer, D. V. (2019). Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing. STEM CELLS, 37(12), 1556-1566. https://doi.org/10.1002/stem.3096

Harvard

Bao, X, Adil, MM, Muckom, R, Zimmermann, JA, Tran, A, Suhy, N, Xu, Y, Sampayo, RG, Clark, DS & Schaffer, DV 2019, 'Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing', STEM CELLS, vol. 37, no. 12, pp. 1556-1566. https://doi.org/10.1002/stem.3096