Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of California at Irvine
  • University of California at Los Angeles
  • University of Pittsburgh
  • California Institute of Technology
  • Massachusetts Institute of Technology
  • Louisiana State University

Details

Original languageEnglish (US)
Article number1944
JournalNature communications
Volume10
Issue number1
StatusPublished - Dec 1 2019
Peer-reviewedYes

Abstract

Heart rate is under the precise control of the autonomic nervous system. However, the wiring of peripheral neural circuits that regulate heart rate is poorly understood. Here, we develop a clearing-imaging-analysis pipeline to visualize innervation of intact hearts in 3D and employed a multi-technique approach to map parasympathetic and sympathetic neural circuits that control heart rate in mice. We identify cholinergic neurons and noradrenergic neurons in an intrinsic cardiac ganglion and the stellate ganglia, respectively, that project to the sinoatrial node. We also report that the heart rate response to optogenetic versus electrical stimulation of the vagus nerve displays different temporal characteristics and that vagal afferents enhance parasympathetic and reduce sympathetic tone to the heart via central mechanisms. Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs.

Citation formats

APA

Rajendran, P. S., Challis, R. C., Fowlkes, C. C., Hanna, P., Tompkins, J. D., Jordan, M. C., ... Shivkumar, K. (2019). Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Nature communications, 10(1), [1944]. https://doi.org/10.1038/s41467-019-09770-1

Harvard

Rajendran, PS, Challis, RC, Fowlkes, CC, Hanna, P, Tompkins, JD, Jordan, MC, Hiyari, S, Gabris-Weber, BA, Greenbaum, A, Chan, KY, Deverman, BE, Münzberg, H, Ardell, JL, Salama, G, Gradinaru, V & Shivkumar, K 2019, 'Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies', Nature communications, vol. 10, no. 1, 1944. https://doi.org/10.1038/s41467-019-09770-1