Isolation and characterization of microvesicles from mesenchymal stem cells

Research output: Contribution to journalArticle

Authors

  • M. Rezaa Mohammadi
  • Milad Riazifar
  • Egest J. Pone
  • Ashish Yeri
  • Kendall Van Keuren-Jensen
  • Cecilia Lässer
  • Jan Lotvall
  • Weian Zhao

External Institution(s)

  • University of California at Irvine
  • Translational Genomics Research Institute
  • University of Gothenburg

Details

Original languageEnglish (US)
Pages (from-to)50-57
Number of pages8
JournalMethods
Volume177
StatusPublished - May 1 2020
Peer-reviewedYes

Abstract

Mesenchymal stem or stromal cells are currently under clinical investigation for multiple diseases. While their mechanism of action is still not fully elucidated, vesicles secreted by MSCs are believed to recapitulate their therapeutic potentials to some extent. Microvesicles (MVs), also called as microparticles or ectosome, are among secreted vesicles that could transfer cytoplasmic cargo, including RNA and proteins, from emitting (source) cells to recipient cells. Given the importance of MVs, we here attempted to establish a method to isolate and characterize MVs secreted from unmodified human bone marrow derived MSCs (referred to as native MSCs, and their microvesicles as Native-MVs) and IFNγ stimulated MSCs (referred to as IFNγ-MSCs, and their microvesicles as IFNγ-MVs). We first describe an ultracentrifugation technique to isolate MVs from the conditioned cell culture media of MSCs. Next, we describe characterization and quality control steps to analyze the protein and RNA content of MVs. Finally, we examined the potential of MVs to exert immunomodulatory effects through induction of regulatory T cells (Tregs). Secretory vesicles from MSCs are promising alternatives for cell therapy with applications in drug delivery, regenerative medicine, and immunotherapy.

    Research areas

  • Cell-free therapy, Drug delivery, Extracellular vesicles (EVs), Mesenchymal Stem Cells (MSCs), Microvesicles (MVs)

Citation formats

APA

Mohammadi, M. R., Riazifar, M., Pone, E. J., Yeri, A., Van Keuren-Jensen, K., Lässer, C., ... Zhao, W. (2020). Isolation and characterization of microvesicles from mesenchymal stem cells. Methods, 177, 50-57. https://doi.org/10.1016/j.ymeth.2019.10.010

Harvard

Mohammadi, MR, Riazifar, M, Pone, EJ, Yeri, A, Van Keuren-Jensen, K, Lässer, C, Lotvall, J & Zhao, W 2020, 'Isolation and characterization of microvesicles from mesenchymal stem cells', Methods, vol. 177, pp. 50-57. https://doi.org/10.1016/j.ymeth.2019.10.010