Live 4D optical coherence tomography for early embryonic mouse cardiac phenotyping

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Authors

External Institution(s)

  • University of Houston
  • Samara National Research University
  • Baylor College of Medicine

Details

Original languageEnglish (US)
Title of host publicationOptical Methods in Developmental Biology IV
EditorsMichael A. Choma, Scott E. Fraser, Andrew M. Rollins
StatusPublished - Jan 1 2016
EventOptical Methods in Developmental Biology IV - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9716
ISSN (Print)1605-7422

Other

OtherOptical Methods in Developmental Biology IV
CountryUnited States
CitySan Francisco
Period02/13/201602/14/2016

Abstract

Studying embryonic mouse development is important for our understanding of normal human embryogenesis and the underlying causes of congenital defects. Our research focuses on imaging early development in the mouse embryo to specifically understand cardiovascular development using optical coherence tomography (OCT). We have previously developed imaging approaches that combine static embryo culture, OCT imaging and advanced image processing to visualize the whole live mouse embryos and obtain 4D (3D+time) cardiodynamic datasets with cellular resolution. Here, we present the study of using 4D OCT for dynamic imaging of early embryonic heart in live mouse embryos to assess mutant cardiac phenotypes during development, including a cardiac looping defect. Our results indicate that the live 4D OCT imaging approach is an efficient phenotyping tool that can reveal structural and functional cardiac defects at very early stages. Further studies integrating live embryonic cardiodynamic phenotyping with molecular and genetic approaches in mouse mutants will help to elucidate the underlying signaling defects.

    Research areas

  • Wdr19, cardiodynamics, cardiovascular development, embryology, four-dimensional imaging, heart looping, mouse, neural tube closure, optical coherence tomography

Citation formats

APA

Lopez, A., Wang, S., Larin, K. V., Overbeek, P. A., & Larina, I. (2016). Live 4D optical coherence tomography for early embryonic mouse cardiac phenotyping. In M. A. Choma, S. E. Fraser, & A. M. Rollins (Eds.), Optical Methods in Developmental Biology IV [971609] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9716). SPIE. https://doi.org/10.1117/12.2212452

Harvard

Lopez, A, Wang, S, Larin, KV, Overbeek, PA & Larina, I 2016, Live 4D optical coherence tomography for early embryonic mouse cardiac phenotyping. in MA Choma, SE Fraser & AM Rollins (eds), Optical Methods in Developmental Biology IV., 971609, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9716, SPIE, Optical Methods in Developmental Biology IV, San Francisco, United States, 02/13/2016. https://doi.org/10.1117/12.2212452