Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Details

Original languageEnglish (US)
Title of host publicationDiagnosis and Treatment of Diseases in the Breast and Reproductive System IV
EditorsMelissa C. Skala, Paul J. Campagnola
StatusPublished - Jan 1 2018
EventDiagnosis and Treatment of Diseases in the Breast and Reproductive System IV 2018 - San Francisco, United States
Duration: Jan 27 2018Jan 28 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10472
ISSN (Print)1605-7422

Other

OtherDiagnosis and Treatment of Diseases in the Breast and Reproductive System IV 2018
CountryUnited States
CitySan Francisco
Period01/27/201801/28/2018

Abstract

Hemodynamic load, contractile forces, and tissue elasticity are regulators of cardiac development and contribute to the mechanical homeostasis of the developing vertebrate heart. Congenital heart disease (CHD) is a prevalent condition in the United States that affects 8 in 1000 live births[1], and has been linked to disrupted cardiac biomechanics[2-4]. Therefore, it is important to understand how these forces integrate and regulate vertebrate cardiac development to inform clinical strategies to treat CHD early on by reintroducing proper mechanical load or modulating downstream factors that rely on mechanical signalling. Toward investigation of biomechanical regulation of mammalian cardiovascular dynamics and development, our methodology combines live mouse embryo culture protocols, state-of-the-art structural and functional Optical Coherence Tomography (OCT), second harmonic generation (SHG) microscopy, and computational analysis. Using these approaches, we assess functional aspects of the developing heart and characterize how they coincide with a determinant of tissue stiffness and main constituent of the extracellular matrix (ECM) - type I collagen. This work is bringing us closer to understanding how cardiac biomechanics change temporally and spatially during normal development, and how it regulates ECM to maintain mechanical homeostasis for proper function.

    Research areas

  • cardiodynamics, cardiovascular development, collagen, embryology, four-dimensional imaging, mouse, optical coherence tomography, second harmonic generation

Citation formats

APA

Lopez, A. L., Wang, S., & Larina, I. V. (2018). Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography. In M. C. Skala, & P. J. Campagnola (Eds.), Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV [104720S] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10472). SPIE. https://doi.org/10.1117/12.2292104

Harvard

Lopez, AL, Wang, S & Larina, IV 2018, Live dynamic analysis of mouse embryonic cardiogenesis with functional optical coherence tomography. in MC Skala & PJ Campagnola (eds), Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV., 104720S, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10472, SPIE, Diagnosis and Treatment of Diseases in the Breast and Reproductive System IV 2018, San Francisco, United States, 01/27/2018. https://doi.org/10.1117/12.2292104