Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries

Research output: Contribution to journalArticle


External Institution(s)

  • University of Tennessee Health Science Center


Original languageEnglish (US)
Pages (from-to)C1001-C1009
JournalAmerican Journal of Physiology - Cell Physiology
Issue number11
StatusPublished - Jun 1 2016


Anoctamin-1 [ANO1, also known as transmembrane protein 16A (TMEM16A)] is a Ca2+- activated Cl channel expressed in arterial myocytes that regulates membrane potential and contractility. Signaling mechanisms that control ANO1 activity in arterial myocytes are poorly understood. In cerebral artery myocytes, ANO1 channels are activated by local Ca2+ signals generated by plasma membrane nonselective cation channels, but the molecular identity of these proteins is unclear. Arterial myocytes express several different nonselective cation channels, including multiple members of the transient receptor potential receptor (TRP) family. The goal of this study was to identify localized ion channels that control ANO1 currents in cerebral artery myocytes. Coimmunoprecipitation and immunofluorescence resonance energy transfer microscopy experiments indicate that ANO1 and canonical TRP 6 (TRPC6) channels are present in the same macromolecular complex and localize in close spatial proximity in the myocyte plasma membrane. In contrast, ANO1 is not near TRPC3, TRP melastatin 4, or inositol trisphosphate receptor 1 channels. Hyp9, a selective TRPC6 channel activator, stimulated Cl currents in myocytes that were blocked by T16Ainh-A01, an ANO1 inhibitor, ANO1 knockdown using siRNA, and equimolar replacement of intracellular EGTA with BAPTA, a fast Ca2+ chelator that abolishes local Ca2+ signaling. Hyp9 constricted pressurized cerebral arteries, and this response was attenuated by T16Ainh-A01. In contrast, T16Ainh-A01 did not alter depolarization-induced (60 mM K+) vasoconstriction. These data indicate that TRPC6 channels generate a local intracellular Ca2+ signal that activates nearby ANO1 channels in myocytes to stimulate vasoconstriction.

    Research areas

  • Anoctamin-1 channel, Arterial smooth muscle cell, Transient receptor potential channel, Vasoconstriction

Citation formats



Wang, Q, Dennis Leo, M, Narayanan, D, Kuruvilla, KP & Jaggar, JH 2016, 'Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries', American Journal of Physiology - Cell Physiology, vol. 310, no. 11, pp. C1001-C1009. https://doi.org/10.1152/ajpcell.00092.2016