Loss of ADAMTS19 causes progressive non-syndromic heart valve disease

Research output: Contribution to journalLetter

Authors

  • MIBAVA Leducq Consortium principal investigators

External Institution(s)

  • University of Montreal
  • University of Münster
  • Hadassah University Medical Centre
  • Jackson Laboratory
  • Institut national de la santé et de la recherche médicale
  • Kiel University
  • German Centre for Cardiovascular Research
  • Wellcome Sanger Institute
  • Cincinnati Children's Hospital Medical Center
  • Aix Marseille Universite
  • Johns Hopkins University
  • Howard Hughes Medical Institute
  • University of Antwerp
  • Radboud University Nijmegen
  • Karolinska Institutet
  • Universitätsklinikum Schleswig-Holstein Campus Lübeck
  • University of Toronto

Details

Original languageEnglish (US)
Pages (from-to)40-47
Number of pages8
JournalNature Genetics
Volume52
Issue number1
StatusPublished - Jan 1 2020
Peer-reviewedYes

Abstract

Valvular heart disease is observed in approximately 2% of the general population1. Although the initial observation is often localized (for example, to the aortic or mitral valve), disease manifestations are regularly observed in the other valves and patients frequently require surgery. Despite the high frequency of heart valve disease, only a handful of genes have so far been identified as the monogenic causes of disease2–7. Here we identify two consanguineous families, each with two affected family members presenting with progressive heart valve disease early in life. Whole-exome sequencing revealed homozygous, truncating nonsense alleles in ADAMTS19 in all four affected individuals. Homozygous knockout mice for Adamts19 show aortic valve dysfunction, recapitulating aspects of the human phenotype. Expression analysis using a lacZ reporter and single-cell RNA sequencing highlight Adamts19 as a novel marker for valvular interstitial cells; inference of gene regulatory networks in valvular interstitial cells positions Adamts19 in a highly discriminatory network driven by the transcription factor lymphoid enhancer-binding factor 1 downstream of the Wnt signaling pathway. Upregulation of endocardial Krüppel-like factor 2 in Adamts19 knockout mice precedes hemodynamic perturbation, showing that a tight balance in the Wnt–Adamts19–Klf2 axis is required for proper valve maturation and maintenance.

Citation formats

APA

MIBAVA Leducq Consortium principal investigators (2020). Loss of ADAMTS19 causes progressive non-syndromic heart valve disease. Nature Genetics, 52(1), 40-47. https://doi.org/10.1038/s41588-019-0536-2

Harvard

MIBAVA Leducq Consortium principal investigators 2020, 'Loss of ADAMTS19 causes progressive non-syndromic heart valve disease', Nature Genetics, vol. 52, no. 1, pp. 40-47. https://doi.org/10.1038/s41588-019-0536-2