Mammalian TRP ion channels are insensitive to membrane stretch

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • Victor Chang Cardiac Research Institute
  • University of Newcastle
  • Yale University
  • University of New South Wales

Details

Original languageEnglish (US)
JournalJournal of cell science
Volume132
Issue number23
StatusPublished - Jan 1 2019
Peer-reviewedYes

Abstract

TRP channels of the transient receptor potential ion channel superfamily are involved in a wide variety of mechanosensory processes, including touch sensation, pain, blood pressure regulation, bone loading and detection of cerebrospinal fluid flow. However, in many instances it is unclear whether TRP channels are the primary transducers of mechanical force in these processes. In this study, we tested stretch activation of eleven TRP channels from six mammalian subfamilies. We found that these TRP channels were insensitive to short membrane stretches in cellular systems. Furthermore, we purified TRPC6 and demonstrated its insensitivity to stretch in liposomes, an artificial bilayer system free from cellular components. Additionally, we demonstrated that, when expressed in C. elegans neurons, mouse TRPC6 restores the mechanoresponse of a touch insensitive mutant but requires diacylglycerol for activation. These results strongly suggest that the mammalian members of the TRP ion channel family are insensitive to tension induced by cell membrane stretching and, thus, are more likely to be activated by cytoplasmic tethers or downstream components and to act as amplifiers of cellular mechanosensory signaling cascades.

    Research areas

  • Ion channel reconstitution, Mechanosensitive channels, Mechanotransduction, TRP ion channels, TRPC6

Citation formats

APA

Nikolaev, Y. A., Cox, C. D., Ridone, P., Rohde, P. R., Cordero-Morales, J. F., Vásquez, V., ... Martinac, B. (2019). Mammalian TRP ion channels are insensitive to membrane stretch. Journal of cell science, 132(23). https://doi.org/10.1242/jcs.238360

Harvard

Nikolaev, YA, Cox, CD, Ridone, P, Rohde, PR, Cordero-Morales, JF, Vásquez, V, Laver, DR & Martinac, B 2019, 'Mammalian TRP ion channels are insensitive to membrane stretch', Journal of cell science, vol. 132, no. 23. https://doi.org/10.1242/jcs.238360