Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics

Research output: Contribution to journalArticle

Authors

  • Guoxia Liu
  • Arianne Papa
  • Alexander N. Katchman
  • Sergey I. Zakharov
  • Daniel Roybal
  • Jessica A. Hennessey
  • Jared Kushner
  • Lin Yang
  • Bi Xing Chen
  • Alexander Kushnir
  • Katerina Dangas
  • Steven P. Gygi
  • Geoffrey S. Pitt
  • Henry M. Colecraft
  • Manu Ben-Johny
  • Marian Kalocsay
  • Steven O. Marx

External Institution(s)

  • Columbia University
  • Harvard University
  • Cornell University

Details

Original languageEnglish (US)
Pages (from-to)695-700
Number of pages6
JournalNature
Volume577
Issue number7792
StatusPublished - Jan 30 2020
Peer-reviewedYes

Abstract

Increased cardiac contractility during the fight-or-flight response is caused by β-adrenergic augmentation of CaV1.2 voltage-gated calcium channels1–4. However, this augmentation persists in transgenic murine hearts expressing mutant CaV1.2 α1C and β subunits that can no longer be phosphorylated by protein kinase A—an essential downstream mediator of β-adrenergic signalling—suggesting that non-channel factors are also required. Here we identify the mechanism by which β-adrenergic agonists stimulate voltage-gated calcium channels. We express α1C or β2B subunits conjugated to ascorbate peroxidase5 in mouse hearts, and use multiplexed quantitative proteomics6,7 to track hundreds of proteins in the proximity of CaV1.2. We observe that the calcium-channel inhibitor Rad8,9, a monomeric G protein, is enriched in the CaV1.2 microenvironment but is depleted during β-adrenergic stimulation. Phosphorylation by protein kinase A of specific serine residues on Rad decreases its affinity for β subunits and relieves constitutive inhibition of CaV1.2, observed as an increase in channel open probability. Expression of Rad or its homologue Rem in HEK293T cells also imparts stimulation of CaV1.3 and CaV2.2 by protein kinase A, revealing an evolutionarily conserved mechanism that confers adrenergic modulation upon voltage-gated calcium channels.

Citation formats

APA

Liu, G., Papa, A., Katchman, A. N., Zakharov, S. I., Roybal, D., Hennessey, J. A., ... Marx, S. O. (2020). Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics. Nature, 577(7792), 695-700. https://doi.org/10.1038/s41586-020-1947-z

Harvard

Liu, G, Papa, A, Katchman, AN, Zakharov, SI, Roybal, D, Hennessey, JA, Kushner, J, Yang, L, Chen, BX, Kushnir, A, Dangas, K, Gygi, SP, Pitt, GS, Colecraft, HM, Ben-Johny, M, Kalocsay, M & Marx, SO 2020, 'Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics', Nature, vol. 577, no. 7792, pp. 695-700. https://doi.org/10.1038/s41586-020-1947-z