Mechanism of modulation of AMPA receptors by TARP-γ8

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Texas Health Science Center at Houston
  • Cornell University

Details

Original languageEnglish (US)
JournalJournal of General Physiology
Volume152
Issue number1
StatusPublished - Jan 6 2020
Peer-reviewedYes

Abstract

Fast excitatory synaptic transmission in the mammalian central nervous system is mediated by glutamate-activated α-amino-5-methyl-3-hydroxy-4-isoxazole propionate (AMPA) receptors. In neurons, AMPA receptors coassemble with transmembrane AMPA receptor regulatory proteins (TARPs). Assembly with TARP γ8 alters the biophysical properties of the receptor, producing resensitization currents in the continued presence of glutamate. Using single-channel recordings, we show that under resensitizing conditions, GluA2 AMPA receptors primarily transition to higher conductance levels, similar to activation of the receptors in the presence of cyclothiazide, which stabilizes the open state. To study the conformation associated with these states, we have used single-molecule FRET and show that this high-conductance state exhibits tighter coupling between subunits in the extracellular parts of the receptor. Furthermore, the dwell times for the transition from the tightly coupled state to the decoupled states correlate to longer open durations of the channels, thus correlating conformation and function at the single-molecule level.

Citation formats

APA

Carrillo, E., Shaikh, S. A., Berka, V., Durham, R. J., Litwin, D. B., Lee, G., ... Jayaraman, V. (2020). Mechanism of modulation of AMPA receptors by TARP-γ8. Journal of General Physiology, 152(1). https://doi.org/10.1085/JGP.201912451

Harvard

Carrillo, E, Shaikh, SA, Berka, V, Durham, RJ, Litwin, DB, Lee, G, MacLean, DM, Nowak, LM & Jayaraman, V 2020, 'Mechanism of modulation of AMPA receptors by TARP-γ8', Journal of General Physiology, vol. 152, no. 1. https://doi.org/10.1085/JGP.201912451