Metformin-encapsulated liposome delivery system: An effective treatment approach against breast cancer

Research output: Contribution to journalArticle

Authors

  • Snehal K. Shukla
  • Nishant S. Kulkarni
  • Amanda Chan
  • Vineela Parvathaneni
  • Pamela Farrales
  • Aaron Muth
  • Vivek Gupta

External Institution(s)

  • St. John's University

Details

Original languageEnglish (US)
Article number559
JournalPharmaceutics
Volume11
Issue number11
StatusPublished - Nov 2019
Peer-reviewedYes

Abstract

This study aimed at developing metformin hydrochloride (Met) encapsulated liposomal vesicles for enhanced therapeutic outcomes at reduced doses against breast cancer. Liposomal Met was prepared using thin-film hydration through various loading methods; passive loading, active loading, and drug-loaded lipid film. The drug-loaded film method exhibited maximum entrapment efficiency (~65%) as compared to active loading (~25%) and passive loading (~5%) prepared Met-loaded liposomes. The therapeutic efficacy of these optimized liposomes was evaluated for cellular uptake, cytotoxicity, inhibition of metastatic activity, and apoptosis-inducing activity. Results demonstrated significantly superior activity of positively charged liposomes resulting in reduced IC50 values, minimal cell migration activity, reduced colony formation, and profound apoptosis-induced activity in breast cancer cells as compared to Met. The anti-tumor activity was investigated using a clinically relevant in vitro tumor simulation model, which confirmed enhanced anti-tumorigenic property of liposomal Met over Met itself. To the authors’ knowledge, this is the first report of Met-loaded liposomes for improving the efficacy and therapeutic effect of Met against breast cancer. With the results obtained, it can be speculated that liposomal encapsulation of metformin offers a potentially promising and convenient approach for enhanced efficacy and bioavailability in breast cancer treatment.

    Research areas

  • 3D spheroids, AMPK activation, Breast cancer, Liposomes, Metformin

Citation formats

APA

Shukla, S. K., Kulkarni, N. S., Chan, A., Parvathaneni, V., Farrales, P., Muth, A., & Gupta, V. (2019). Metformin-encapsulated liposome delivery system: An effective treatment approach against breast cancer. Pharmaceutics, 11(11), [559]. https://doi.org/10.3390/pharmaceutics11110559

Harvard

Shukla, SK, Kulkarni, NS, Chan, A, Parvathaneni, V, Farrales, P, Muth, A & Gupta, V 2019, 'Metformin-encapsulated liposome delivery system: An effective treatment approach against breast cancer', Pharmaceutics, vol. 11, no. 11, 559. https://doi.org/10.3390/pharmaceutics11110559