Motor deficit in the mouse ferric chloride-induced distal middle cerebral artery occlusion model of stroke

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • Texas Tech University Health Sciences Center
  • Yerevan State Medical University

Details

Original languageEnglish (US)
Article number112418
JournalBehavioural Brain Research
Volume380
StatusPublished - Feb 17 2020
Peer-reviewedYes

Abstract

Ferric chloride-induced distal middle cerebral artery occlusion (MCAO) model of stroke was described in mice several years ago, however it lacked in-depth evaluation of the post-stroke functional outcomes in the animals. In this study, we reproduced the recently developed model and expanded its characterization by thorough evaluation of blood supply, cerebral infarction, and motor function in adult male and female mice up to 14 days after stroke. Our observations indicate near complete interruption of blood flow in the distal MCA shortly after application of 20 % ferric chloride over the artery through a cranial window, which remained occluded for at least 4 h. As expected, infarction of the brain tissue, documented by TTC and hematoxylin stains, was restricted to the cerebral cortex. We also systematically evaluated motor impairment of the animals in this model. For this, a series of studies were carried out in male and female mice up to 14 days after stroke, and motor function was assessed in cylinder and grid-walking tests in blinded manner. Contrary to our expectations, the results of both motor tests indicated minor, transient motor deficit in mice after stroke. Based on these observations, we conclude that the mouse ferric chloride-induced distal MCAO model is likely not suitable for proof-of-concept and preclinical studies where motor function is an important outcome measure.

    Research areas

  • Cylinder test, FeCl-induced distal MCAO model of stroke, Grid-walking test, Motor impairment, Mouse stroke model

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