Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro

Research output: Contribution to journalArticle

Authors

  • Andrew T. Lombardo
  • Shane R. Nelson
  • M. Yusuf Ali
  • Guy G. Kennedy
  • Kathleen M. Trybus
  • Sam Walcott
  • David M. Warshaw

External Institution(s)

  • University of Vermont
  • University of California at Davis

Details

Original languageEnglish (US)
Article number15692
JournalNature communications
Volume8
StatusPublished - Jun 1 2017
Peer-reviewedYes

Abstract

Intracellular cargo transport relies on myosin Va molecular motor ensembles to travel along the cell's three-dimensional (3D) highway of actin filaments. At actin filament intersections, the intersecting filament is a structural barrier to and an alternate track for directed cargo transport. Here we use 3D super-resolution fluorescence imaging to determine the directional outcome (that is, continues straight, turns or terminates) for an -1/410 motor ensemble transporting a 350 nm lipid-bound cargo that encounters a suspended 3D actin filament intersection in vitro. Motor-cargo complexes that interact with the intersecting filament go straight through the intersection 62% of the time, nearly twice that for turning. To explain this, we develop an in silico model, supported by optical trapping data, suggesting that the motors' diffusive movements on the vesicle surface and the extent of their engagement with the two intersecting actin tracks biases the motor-cargo complex on average to go straight through the intersection.

Citation formats

APA

Lombardo, A. T., Nelson, S. R., Ali, M. Y., Kennedy, G. G., Trybus, K. M., Walcott, S., & Warshaw, D. M. (2017). Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro. Nature communications, 8, [15692]. https://doi.org/10.1038/ncomms15692

Harvard

Lombardo, AT, Nelson, SR, Ali, MY, Kennedy, GG, Trybus, KM, Walcott, S & Warshaw, DM 2017, 'Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro', Nature communications, vol. 8, 15692. https://doi.org/10.1038/ncomms15692