New mouse model of chronic kidney disease transitioned from ischemic acute kidney injury

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of South Florida

Details

Original languageEnglish (US)
Pages (from-to)F286-F295
JournalAmerican Journal of Physiology - Renal Physiology
Volume317
Issue number2
StatusPublished - Aug 2019
Peer-reviewedYes

Abstract

Acute kidney injury (AKI) significantly increases the risk of development of chronic kidney disease (CKD), which is closely associated with the severity of AKI. However, the underlying mechanisms for the AKI to CKD transition remain unclear. Several animal models with AKI to CKD transition have been generated and widely used in research; however, none of them exhibit the typical changes in glomerular filtration rate or plasma creatinine, the hallmarks of CKD. In the present study, we developed a novel model with a typical phenotype of AKI to CKD transition in C57BL/6 mice. In this model, life-threatening ischemia-reperfusion injury was performed in one kidney, whereas the contralateral kidney was kept intact to maintain animal survival; then, after 2 wk of recovery, when the renal function of the injured kidney restored above the survival threshold, the contralateral intact kidney was removed. Animals of this two-stage unilateral ischemia-reperfusion injury model with pedicle clamping of 21 and 24 min exhibited an incomplete recovery from AKI and subsequent progression of CKD with characteristics of a progressive decline in glomerular filtration rate, increase in plasma creatinine, worsening of proteinuria, and deleterious histopathological changes, including interstitial fibrosis and glomerulosclerosis. In conclusion, a new model of the AKI to CKD transition was generated in C57BL/6 mice.

    Research areas

  • Acute kidney injury, Animal model, Chronic kidney disease, Ischemia-reperfusion injury

Citation formats

APA

Wei, J., Zhang, J., Wang, L., Jiang, S., Fu, L., Buggs, J., & Liu, R. (2019). New mouse model of chronic kidney disease transitioned from ischemic acute kidney injury. American Journal of Physiology - Renal Physiology, 317(2), F286-F295. https://doi.org/10.1152/ajprenal.00021.2019

Harvard

Wei, J, Zhang, J, Wang, L, Jiang, S, Fu, L, Buggs, J & Liu, R 2019, 'New mouse model of chronic kidney disease transitioned from ischemic acute kidney injury', American Journal of Physiology - Renal Physiology, vol. 317, no. 2, pp. F286-F295. https://doi.org/10.1152/ajprenal.00021.2019