Non-invasive tracking of CD4 + T cells with a paramagnetic and fluorescent nanoparticle in brain ischemia

Research output: Contribution to journalArticle

Authors

  • Wei Na Jin
  • Xiaoxia Yang
  • Zhiguo Li
  • Minshu Li
  • Samuel Xiang Yu Shi
  • Kristofer Wood
  • Qingwei Liu
  • Ying Fu
  • Wei Han
  • Yun Xu
  • Fu Dong Shi
  • Qiang Liu

External Institution(s)

  • Tianjin Medical University
  • St. Joseph's Hospital and Medical Center
  • Nanjing University

Details

Original languageEnglish (US)
Pages (from-to)1464-1476
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume36
Issue number8
StatusPublished - Aug 1 2016
Peer-reviewedYes

Abstract

Recent studies have demonstrated that lymphocytes play a key role in ischemic brain injury. However, there is still a lack of viable approaches to non-invasively track infiltrating lymphocytes and reveal their key spatiotemporal events in the inflamed central nervous system (CNS). Here we describe an in vivo imaging approach for sequential monitoring of brain-infiltrating CD4 + T cells in experimental ischemic stroke. We show that magnetic resonance imaging (MRI) or Xenogen imaging combined with labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4 + T cells in a passive transfer model. MIRB-labeled CD4 + T cells can be longitudinally visualized in the mouse brain and peripheral organs such as the spleen and liver after cerebral ischemia. Immunostaining of tissue sections showed similar kinetics of MIRB-labeled CD4 + T cells when compared with in vivo observations. Our results demonstrated the use of MIRB coupled with in vivo imaging as a valid method to track CD4 + T cells in ischemic brain injury. This approach will facilitate future investigations to identify the dynamics and key spatiotemporal events for brain-infiltrating lymphocytes in CNS inflammatory diseases.

    Research areas

  • CD4 + T cells, In vivo imaging, Ischemic stroke, USPIO, lymphocytes

Citation formats

APA

Jin, W. N., Yang, X., Li, Z., Li, M., Shi, S. X. Y., Wood, K., ... Liu, Q. (2016). Non-invasive tracking of CD4 + T cells with a paramagnetic and fluorescent nanoparticle in brain ischemia. Journal of Cerebral Blood Flow and Metabolism, 36(8), 1464-1476. https://doi.org/10.1177/0271678X15611137

Harvard

Jin, WN, Yang, X, Li, Z, Li, M, Shi, SXY, Wood, K, Liu, Q, Fu, Y, Han, W, Xu, Y, Shi, FD & Liu, Q 2016, 'Non-invasive tracking of CD4 + T cells with a paramagnetic and fluorescent nanoparticle in brain ischemia', Journal of Cerebral Blood Flow and Metabolism, vol. 36, no. 8, pp. 1464-1476. https://doi.org/10.1177/0271678X15611137