Novel Role for the AnxA1-Fpr2/ALX Signaling Axis as a Key Regulator of Platelet Function to Promote Resolution of Inflammation

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • LSU Health Sciences Center - Shreveport
  • University of Münster
  • Pathology and Translational Pathobiology
  • INLET
  • Baker Heart Research Institute
  • Departments of Neurosurgery
  • Columbia University
  • Queen Mary University of London

Details

Original languageEnglish (US)
Pages (from-to)319-335
Number of pages17
JournalCirculation
Volume140
Issue number4
StatusPublished - Jul 23 2019
Peer-reviewedYes

Abstract

Background: Ischemia reperfusion injury (I/RI) is a common complication of cardiovascular diseases. Resolution of detrimental I/RI-generated prothrombotic and proinflammatory responses is essential to restore homeostasis. Platelets play a crucial part in the integration of thrombosis and inflammation. Their role as participants in the resolution of thromboinflammation is underappreciated; therefore we used pharmacological and genetic approaches, coupled with murine and clinical samples, to uncover key concepts underlying this role. Methods: Middle cerebral artery occlusion with reperfusion was performed in wild-type or annexin A1 (AnxA1) knockout (AnxA1-/-) mice. Fluorescence intravital microscopy was used to visualize cellular trafficking and to monitor light/dye-induced thrombosis. The mice were treated with vehicle, AnxA1 (3.3 mg/kg), WRW4 (1.8 mg/kg), or all 3, and the effect of AnxA1 was determined in vivo and in vitro. Results: Intravital microscopy revealed heightened platelet adherence and aggregate formation post I/RI, which were further exacerbated in AnxA1-/- mice. AnxA1 administration regulated platelet function directly (eg, via reducing thromboxane B2 and modulating phosphatidylserine expression) to promote cerebral protection post-I/RI and act as an effective preventative strategy for stroke by reducing platelet activation, aggregate formation, and cerebral thrombosis, a prerequisite for ischemic stroke. To translate these findings into a clinical setting, we show that AnxA1 plasma levels are reduced in human and murine stroke and that AnxA1 is able to act on human platelets, suppressing classic thrombin-induced inside-out signaling events (eg, Akt activation, intracellular calcium release, and Ras-associated protein 1 [Rap1] expression) to decrease IIbβ3 activation without altering its surface expression. AnxA1 also selectively modifies cell surface determinants (eg, phosphatidylserine) to promote platelet phagocytosis by neutrophils, thereby driving active resolution. (n=5-13 mice/group or 7-10 humans/group.) Conclusions: AnxA1 affords protection by altering the platelet phenotype in cerebral I/RI from propathogenic to regulatory and reducing the propensity for platelets to aggregate and cause thrombosis by affecting integrin (IIbβ3) activation, a previously unknown phenomenon. Thus, our data reveal a novel multifaceted role for AnxA1 to act both as a therapeutic and a prophylactic drug via its ability to promote endogenous proresolving, antithromboinflammatory circuits in cerebral I/RI. Collectively, these results further advance our knowledge and understanding in the field of platelet and resolution biology.

    Research areas

  • Annexin A1, formyl peptide receptor, inflammation, integrins, stroke, thrombosis

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Senchenkova, EY, Ansari, J, Becker, F, Vital, SA, Al-Yafeai, Z, Sparkenbaugh, EM, Pawlinski, R, Stokes, KY, Carroll, JL, Dragoi, AM, Qin, CX, Ritchie, RH, Sun, H, Cuellar-Saenz, HH, Rubinstein, MR, Han, YW, Orr, AW, Perretti, M, Granger, DN & Gavins, FNE 2019, 'Novel Role for the AnxA1-Fpr2/ALX Signaling Axis as a Key Regulator of Platelet Function to Promote Resolution of Inflammation', Circulation, vol. 140, no. 4, pp. 319-335. https://doi.org/10.1161/CIRCULATIONAHA.118.039345