p53-cofactor JMY is a multifunctional actin nucleation factor

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Oxford
  • University of California at Los Angeles
  • University of California at San Francisco

Details

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalNature cell biology
Volume11
Issue number4
StatusPublished - Mar 16 2009
Peer-reviewedYes

Abstract

Many cellular structures are assembled from networks of actin filaments, and the architecture of these networks depends on the mechanism by which the filaments are formed. Several classes of proteins are known to assemble new filaments, including the Arp2/3 complex, which creates branched filament networks, and Spire, which creates unbranched filaments. We find that JMY, a vertebrate protein first identified as a transcriptional co-activator of p53, combines these two nucleating activities by both activating Arp2/3 and assembling filaments directly using a Spire-like mechanism. Increased levels of JMY expression enhance motility, whereas loss of JMY slows cell migration. When slowly migrating HL-60 cells are differentiated into highly motile neutrophil-like cells, JMY moves from the nucleus to the cytoplasm and is concentrated at the leading edge. Thus, JMY represents a new class of multifunctional actin assembly factor whose activity is regulated, at least in part, by sequestration in the nucleus.

Citation formats

APA

Zuchero, J. B., Coutts, A. S., Quinlan, M. E., La Thangue, N. B., & Mullins, R. D. (2009). p53-cofactor JMY is a multifunctional actin nucleation factor. Nature cell biology, 11(4), 451-459. https://doi.org/10.1038/ncb1852

Harvard

Zuchero, JB, Coutts, AS, Quinlan, ME, La Thangue, NB & Mullins, RD 2009, 'p53-cofactor JMY is a multifunctional actin nucleation factor', Nature cell biology, vol. 11, no. 4, pp. 451-459. https://doi.org/10.1038/ncb1852