Regulation of SRF protein stability by an autophagy-dependent pathway

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Rochester
  • Chinese Academy of Medical Sciences

Details

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume521
Issue number2
StatusPublished - Jan 8 2020
Peer-reviewedYes

Abstract

Serum response factor (SRF), a key transcription factor, plays an important role in regulating cell functions such as proliferation and differentiation. Most proteins are unstable, and protein stability is regulated through the ubiquitin-proteasome system (UPS) or the autophagy lysosome pathway (ALP). Whether SRF is degraded and what mechanisms control SRF protein stability remain unexplored. Western blot analyses of cells treated with cycloheximide (CHX), a protein synthesis inhibitor, showed that SRF was degraded in a time-dependent manner. Moreover, we observed that SRF undergoes autophagy-dependent destruction, which is accelerated by serum deprivation. Through bioinformatics screening, we found that SRF contains the GSK3β phosphorylation motif (T/SPPXS): SPDSPPRSDPT, which is conserved from zebrafish to humans. Serum deprivation stimulated GSK3β activation that then potentiates SRF degradation through the autophagy lysosome pathway. Since SRF is important for numerous cellular activities, our results suggest that the autophagy-dependent SRF degradation pathway may provide a new avenue to modulate SRF-mediated cell functions.

    Research areas

  • Autophagy lysosome pathway, Degradation, Serum response factor (SRF)

Citation formats

APA

Luo, J., Jin, F. Q., Yin, M., & Jin, Z. G. (2020). Regulation of SRF protein stability by an autophagy-dependent pathway. Biochemical and Biophysical Research Communications, 521(2), 279-284. https://doi.org/10.1016/j.bbrc.2019.09.104

Harvard

Luo, J, Jin, FQ, Yin, M & Jin, ZG 2020, 'Regulation of SRF protein stability by an autophagy-dependent pathway', Biochemical and Biophysical Research Communications, vol. 521, no. 2, pp. 279-284. https://doi.org/10.1016/j.bbrc.2019.09.104