Regulator of G-Protein Signaling 16 Is a Negative Modulator of Platelet Function and Thrombosis

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Texas at El Paso
  • National Institutes of Health

Details

Original languageEnglish (US)
Article numbere011273
JournalJournal of the American Heart Association
Volume8
Issue number5
StatusPublished - Mar 5 2019
Peer-reviewedYes

Abstract

Background: Members of the regulator of G-protein signaling (RGS) family inhibit G-protein coupled receptor signaling by modulating G-protein activity. In platelets, there are 3 different RGS isoforms that are expressed at the protein level, including RGS16. Recently, we have shown that CXCL12 regulates platelet function via RGS16. However, the role of RGS16 in platelet function and thrombus formation is poorly defined. Methods and Results: We used a genetic knockout mouse model approach to examine the role(s) of RGS16 in platelet activation by using a host of in vitro and in vivo assays. We observed that agonist-induced platelet aggregation, secretion, and integrin activation were much more pronounced in platelets from the RGS16 knockout (Rgs16−/−) mice relative to their wild type (Rgs16+/+) littermates. Furthermore, the Rgs16−/− mice had a markedly shortened bleeding time and were more susceptible to vascular injury–associated thrombus formation than the controls. Conclusions: These findings support a critical role for RGS16 in regulating hemostatic and thrombotic functions of platelets in mice. Hence, RGS16 represents a potential therapeutic target for modulating platelet function.

    Research areas

  • RGS16, hemostasis, platelet, regulator of G-protein signaling, signal transduction, thrombosis

Citation formats

APA

Hernandez, K. R., Karim, Z. A., Qasim, H., Druey, K. M., Alshbool, F. Z., & Khasawneh, F. T. (2019). Regulator of G-Protein Signaling 16 Is a Negative Modulator of Platelet Function and Thrombosis. Journal of the American Heart Association, 8(5), [e011273]. https://doi.org/10.1161/JAHA.118.011273

Harvard

Hernandez, KR, Karim, ZA, Qasim, H, Druey, KM, Alshbool, FZ & Khasawneh, FT 2019, 'Regulator of G-Protein Signaling 16 Is a Negative Modulator of Platelet Function and Thrombosis', Journal of the American Heart Association, vol. 8, no. 5, e011273. https://doi.org/10.1161/JAHA.118.011273