Scavenger receptors of endothelial cells mediate the uptake and cellular proatherogenic effects of carbamylated LDL

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Arkansas for Medical Sciences
  • Department of Veterans Affairs

Details

Original languageEnglish (US)
Pages (from-to)1622-1630
Number of pages9
JournalArteriosclerosis, thrombosis, and vascular biology
Volume29
Issue number10
StatusPublished - Oct 1 2009
Peer-reviewedYes

Abstract

OBJECTIVE-: Carbamylated LDL (cLDL) has been recently shown to have robust proatherogenic effects on human endothelial cells in vitro, suggesting cLDL may have a significant role in atherosclerosis in uremia. The current study was designed to determine which receptors are used by cLDL and thus cause the proatherogenic effects. METHODS AND RESULTS-: In ex vivo or in vitro models as well as in intact animals, administration of cLDL was associated with endothelial internalization of cLDL and subendothelial translocation (transcytosis). In vitro recombinant LOX-1 and SREC-1 receptors showed the greatest cLDL binding. However, pretreatment of the endothelial cells with specific inhibiting antibodies demonstrated that cLDL binds mainly to LOX-1 and CD36 receptors. The transcytosis was dependent on SR-A1, SREC-1, and CD36 receptors whereas LOX-1 receptor was not involved. The cytotoxicity was mediated by several studied scavenger receptors, but cLDL-induced monocyte adhesion depended only on LOX-1. The cLDL-induced synthesis of LOX-1 protein significantly contributed to both cytotoxicity and accelerated monocyte adhesion to endothelial cells. CONCLUSIONS-: Our data suggest that cLDL uses a unique pattern of scavenger receptors. They show that LOX-1 receptor, and partially CD36, SREC-1, and SR-A1 receptors, are essential for the proatherogenic effects of cLDL on human endothelial cells.

    Research areas

  • Atherosclerosis, Carbamylated LDL, Endothelial cells, LOX-1, Scavenger receptor

Citation formats

APA

Apostolov, E. O., Shah, S. V., Ray, D., & Basnakian, A. G. (2009). Scavenger receptors of endothelial cells mediate the uptake and cellular proatherogenic effects of carbamylated LDL. Arteriosclerosis, thrombosis, and vascular biology, 29(10), 1622-1630. https://doi.org/10.1161/ATVBAHA.109.189795

Harvard

Apostolov, EO, Shah, SV, Ray, D & Basnakian, AG 2009, 'Scavenger receptors of endothelial cells mediate the uptake and cellular proatherogenic effects of carbamylated LDL', Arteriosclerosis, thrombosis, and vascular biology, vol. 29, no. 10, pp. 1622-1630. https://doi.org/10.1161/ATVBAHA.109.189795