Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging

Research output: Contribution to journalArticle

Authors

  • Hiroshi Makino
  • Kazuya Hokamura
  • Takahiro Natsume
  • Tetsuro Kimura
  • Yoshinobu Kamio
  • Yasuhiro Magata
  • Hiroki Namba
  • Takasumi Katoh
  • Shigehito Sato
  • Tomoki Hashimoto
  • Kazuo Umemura

External Institution(s)

  • Hamamatsu University School of Medicine
  • Fujita Health University

Details

Original languageEnglish (US)
Pages (from-to)1523-1527
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
Issue number9
StatusPublished - Sep 3 2015
Peer-reviewedYes

Abstract

Serial imaging studies can be useful in characterizing the pathologic and physiologic remodeling of cerebral arteries in various mouse models. We tested the feasibility of using a readily available, conventional 3-T magnetic resonance imaging (MRI) to serially image cerebrovascular remodeling in mice. We utilized a mouse model of intracranial aneurysm as a mouse model of the dynamic, pathologic remodeling of cerebral arteries. Aneurysms were induced by hypertension and a single elastase injection into the cerebrospinal fluid. For the mouse cerebrovascular imaging, we used a conventional 3-T MRI system and a 40-mm saddle coil. We used non-enhanced magnetic resonance angiography (MRA) to detect intracranial aneurysm formation and T2-weighted imaging to detect aneurysmal subarachnoid hemorrhage. A serial MRI was conducted every 2 to 3 days. MRI detection of aneurysm formation and subarachnoid hemorrhage was compared against the postmortem inspection of the brain that was perfused with dye. The imaging times for the MRA and T2-weighted imaging were 3.7±0.5 minutes and 4.8±0.0 minutes, respectively. All aneurysms and subarachnoid hemorrhages were correctly identified by two masked observers on MRI. This MRI-based serial imaging technique was useful in detecting intracranial aneurysm formation and subarachnoid hemorrhage in mice.

    Research areas

  • T2-weighted magnetic resonance imaging (MRI), animal model, intracranial aneurysm, magnetic resonance angiography (MRA), subarachnoid hemorrhage

Citation formats

APA

Makino, H., Hokamura, K., Natsume, T., Kimura, T., Kamio, Y., Magata, Y., ... Umemura, K. (2015). Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging. Journal of Cerebral Blood Flow and Metabolism, 35(9), 1523-1527. https://doi.org/10.1038/jcbfm.2015.78

Harvard

Makino, H, Hokamura, K, Natsume, T, Kimura, T, Kamio, Y, Magata, Y, Namba, H, Katoh, T, Sato, S, Hashimoto, T & Umemura, K 2015, 'Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging', Journal of Cerebral Blood Flow and Metabolism, vol. 35, no. 9, pp. 1523-1527. https://doi.org/10.1038/jcbfm.2015.78