Surgical approach for middle cerebral artery occlusion and reperfusion induced stroke in mice

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • Louisiana State University

Details

Original languageEnglish (US)
Article numbere54302
JournalJournal of Visualized Experiments
Volume2016
Issue number116
StatusPublished - Oct 20 2016
Peer-reviewedYes

Abstract

Stroke is a leading cause of death worldwide and continues to be one of the major causes of long-term adult disabilities. About 87% of strokes are ischemic in origin and occur in the territory of the middle cerebral artery (MCA). Currently the only Food and Drug Administration (FDA) approved drug for the treatment of this devastating disease is tissue plasminogen activator (tPA). However, tPA has a small therapeutic window for administration (3-6 hr), and is only effective in 4% of the patients who actually receive it. Current research focuses on understanding the pathophysiology of stroke in order to find potential therapeutic targets. Thus, reliable models are crucial, and the MCA occlusion (MCAo) model (also termed the intraluminal filament or suture model) is deemed to be the most clinically relevant surgical model of ischemic stroke, and is fairly non-invasive and easily reproducible. Typically the MCAo model is used with rodents, especially with mice due to all the genetic variations available for this species. Here we describe (and present in the video) how to successfully perform the MCAo model (with reperfusion) in mice to generate reliable and reproducible data.

    Research areas

  • Intraluminal filament, Intraluminal suture, Issue 116, Medicine, Middle cerebral artery occlusion, Mouse, Stroke, Transient ischemia reperfusion

Citation formats

APA

Harvard