Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development

Research output: Contribution to journalArticle

Authors

  • Shaohai Fang
  • Jia Li
  • Yang Xiao
  • Minjung Lee
  • Lei Guo
  • Wei Han
  • Tingting Li
  • Matthew C. Hill
  • Tingting Hong
  • William Mo
  • Rang Xu
  • Ping Zhang
  • Fen Wang
  • Jiang Chang
  • Yubin Zhou
  • Deqiang Sun
  • James F. Martin
  • Yun Huang

External Institution(s)

  • Texas A&M University
  • Texas Heart Institute
  • Baylor College of Medicine
  • Shanghai Jiao Tong University

Details

Original languageEnglish (US)
Article number4297
JournalNature communications
Volume10
Issue number1
StatusPublished - Dec 1 2019
Peer-reviewedYes

Abstract

Tet-mediated DNA demethylation plays an important role in shaping the epigenetic landscape and chromatin accessibility to control gene expression. While several studies demonstrated pivotal roles of Tet in regulating embryonic development, little is known about their functions in heart development. Here we analyze DNA methylation and hydroxymethylation dynamics during early cardiac development in both human and mice. We find that cardiac-specific deletion of Tet2 and Tet3 in mice (Tet2/3-DKO) leads to ventricular non-compaction cardiomyopathy (NCC) with embryonic lethality. Single-cell RNA-seq analyses reveal a reduction in cardiomyocyte numbers and transcriptional reprogramming in cardiac tissues upon Tet2/3 depletion. Impaired DNA demethylation and reduced chromatin accessibility in Tet2/3-DKO mice further compromised Ying-yang1 (YY1) binding to its genomic targets, and perturbed high-order chromatin organization at key genes involved in heart development. Our studies provide evidence of the physiological role of Tet in regulating DNA methylation dynamics and chromatin organization during early heart development.

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Fang, S, Li, J, Xiao, Y, Lee, M, Guo, L, Han, W, Li, T, Hill, MC, Hong, T, Mo, W, Xu, R, Zhang, P, Wang, F, Chang, J, Zhou, Y, Sun, D, Martin, JF & Huang, Y 2019, 'Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development', Nature communications, vol. 10, no. 1, 4297. https://doi.org/10.1038/s41467-019-12325-z