The ATPase cycle of human muscle myosin II isoforms: Adaptation of a single mechanochemical cycle for different physiological roles

Research output: Contribution to journalArticle

Authors

External Institution(s)

  • University of Kent
  • University of Kragujevac
  • Illinois Institute of Technology
  • University of Colorado Boulder

Details

Original languageEnglish (US)
Pages (from-to)14267-14278
Number of pages12
JournalJournal of Biological Chemistry
Volume294
Issue number39
StatusPublished - Sep 27 2019
Peer-reviewedYes

Abstract

Striated muscle myosins are encoded by a large gene family in all mammals, including humans. These isoforms define several of the key characteristics of the different striated muscle fiber types, including maximum shortening velocity. We have previously used recombinant isoforms of the motor domains of seven different human myosin isoforms to define the actinmyosin cross-bridge cycle in solution. Here, we present data on an eighth isoform, the perinatal, which has not previously been characterized. The perinatal is distinct from the embryonic isoform, appearing to have features in common with the adult fast-muscle isoforms, including weak affinity of ADP for actinmyosin and fastADPrelease.Wego on to use a recently developed modeling approach, MUSICO, to explore how well the experimentally defined cross-bridge cycles for each isoform in solution can predict the characteristics of muscle fiber contraction, including duty ratio, shortening velocity, ATP economy, and load dependence of these parameters. The work shows that the parameters of the cross-bridge cycle predict many of the major characteristics of each muscle fiber type and raises the question of what sequence changes are responsible for these characteristics.

Citation formats

APA

Johnson, C. A., Walklate, J., Svicevic, M., Mijailovich, S. M., Vera, C., Karabina, A., ... Geeves, M. A. (2019). The ATPase cycle of human muscle myosin II isoforms: Adaptation of a single mechanochemical cycle for different physiological roles. Journal of Biological Chemistry, 294(39), 14267-14278. https://doi.org/10.1074/jbc.RA119.009825

Harvard

Johnson, CA, Walklate, J, Svicevic, M, Mijailovich, SM, Vera, C, Karabina, A, Leinwand, LA & Geeves, MA 2019, 'The ATPase cycle of human muscle myosin II isoforms: Adaptation of a single mechanochemical cycle for different physiological roles', Journal of Biological Chemistry, vol. 294, no. 39, pp. 14267-14278. https://doi.org/10.1074/jbc.RA119.009825