Using C. elegans to study the effects of toxins in sensory ion channels in vivo

Research output: Chapter in Book/Report/Conference proceedingChapter

Details

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
StatusPublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2068
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

Caenorhabditis elegans is a powerful animal model in which transgenesis, behavior, and physiology can be merged to study in vivo the effect of natural and synthetic agonists in sensory ion channels. Worms have polymodal sensory neurons (like the ASH pair) that couple ion channel activation with a robust and easily scorable aversive-like behavior. We expressed the transient receptor potential vanilloid 1 (TRPV1) channel from rat (r) in worms’ ASH neurons and determined its sensitivity to the tarantula double-knot toxin (DkTx) and the active component of chili peppers (capsaicin). This chapter describes protocols for generating and maintaining transgenic rTRPV1 worms to determine dose-dependent behavior. The goal is to provide an efficient tool to characterize the function of sensory channels (wild type and mutants) in vivo.

    Research areas

  • Behavior, C. elegans, Capsaicin, DkTx, Pain, Sensory ion channels, TRPV1, Toxins, Transient receptor potential channels, Worms

Citation formats

APA

Vásquez, V. (2020). Using C. elegans to study the effects of toxins in sensory ion channels in vivo. In Methods in Molecular Biology (pp. 225-238). (Methods in Molecular Biology; Vol. 2068). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9845-6_12

Harvard

Vásquez, V 2020, Using C. elegans to study the effects of toxins in sensory ion channels in vivo. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2068, Humana Press Inc., pp. 225-238. https://doi.org/10.1007/978-1-4939-9845-6_12